All League One and Two sides enter the FA Cup at this stage of the competition whilst the Premier League and Championship have to wait until Round 3 to decide their fate.
Locally, fans based in South Yorkshire will naturally have their eye on their teams, Rotherham United have been handed a rare Yorkshire derby with York City at the Don Valley Stadium; the last time these two met in the league was during the 1999/00 campaign - the Millers won 2-1.
Chesterfield travel to Harrow Borough where a shock home win could be on the cards; for neutrals the possibility of the underdog winning is what sets the FA Cup apart from any competition in the World; you just pray that it doesn't happen to you!
One other Cup shock that I'm sure all Blades will be looking for is on Sunday when the BBC screens the live performance of Sheffield Wednesday - they lost 2-0 at home to Huddersfield Town last Tuesday night, slipping to tenth in the league in what was their game without a win.
The Owls, who are FA Cup winners on three occasions travel to Southport who, despite being without first choice keeper Tony McMillan through suspension aim to pull off a cup shock against a side that is quite possibly the biggest outfit at this stage in the competition.
However it's FC United of Manchester who kick-off proceedings as they travel to Rochdale on Friday night; it's their first ever outing at the first round stage and no doubt they'll be itching to make it all the way to round three and land a possible dream tie against rivals Manchester United.
Many headlines will be made this weekend and many dreams will be shattered, those who play outside the Football League may be happy to just be in the competition at this stage.
But spare a thought for Sheffield FC - the longest serving professional club have yet to make it to this round in their long history; they were knocked out in a replay at Tipton in the last qualifying round; Tipton travel to Carlisle United this Saturday.
:: FA Cup First Round Ties ::
Rochdale vs. FC United of Manchester
Harrow Borough vs. Chesterfield
AFC Wimbledon vs. Ebbsfleet United
Accrington Stanley vs. Oldham Athletic
Barnet vs. Charlton Athletic
AFC Bournemouth vs. Tranmere Rovers
Brentford vs. Aldershot
Brighton & Hove Albion vs. Woking
Bury vs. Exeter City
Carlisle United vs. Tipton Town
Chelmsford vs. Hendon
Cheltenham Town vs. Morecambe
Colchester United vs. Bradford City
Corby vs. Luton Town
Dagenham & Redbridge vs. Leyton Orient
Darlington vs. Bristol Rovers
Dartford vs. Port Vale
Fleetwood Town vs. Walsall
Forest Green Rovers vs. Northampton Town
Gillingham vs. Dover Athletic
Guisley vs. Crawley Town
Hartlepool United vs. Vauxhall Motors
Havant & Waterlooville vs. Droylsden
Hayes & Yeading vs. Wycombe Wanderers
Hereford United vs. Hythe Town
Lincoln City vs. Nuneaton Borough
Macclesfield Town vs. Southend United
Mansfield Town vs. Torquay United
Nott's County vs. Gateshead
Plymouth Argyle vs. Swindon Town
Rotherham United vs. York City
Rushden & Diamonds vs. Yeovil Town
Southampton vs. Shrewsbury Town
Stevenage Borough vs. MK Dons
Stockport County vs. Peterborough United
Swindon Supermarine vs. Eastwood Town
Tamworth vs. Crewe Alexandra
Cambridge United vs. Huddersfield Town
Southport vs. Sheffield Wednesday
Burton Albion vs. Oxford United