Oldham Athletic's James Wilson (right) and Blackpool's Danny Philliskirk battle for the ball.
Wilson, 27 joins the Blades on a free transfer, signing a two year deal following the expiration of his contract at Oldham Athletic where he played 85 games in two years. He becomes Wilder's third signing, joining winger Mark Duffy (Birmingham City) and fellow defender Chris Hussey (Bury) to Bramall Lane.
"James is an athletic, mobile centre-half and knows this division," Blades boss Chris Wilder told sufc.co.uk.
Wilson started his career with Bristol City prior to his move to Boundary Park, he has also played in red and white stripes for Brentford and Cheltenham Town in loan spells.